Summer Training Course on RNA-seq and ChIP-seq data Analysis

This course will be held at University of Aveiro, from 8th to 11th July. The aim of this summer training course is to provide the practical skills to perform integrative analysis of RNA-seq and ChIP-seq data. A case study consisting in the role of histone modifications in the regulation of gene expression as a response to temperature in marine phytoplankton will be used to introduce the different theoretical aspects and software tools.

Directed to: PhD students, post-docs, researchers and staff.

Programme:
• Unit 1: Introduction to R and UNIX: Rstudio and MobaXterm. Introduction to high throughput sequencing: RNA-seq and ChIP-seq data.
• Unit 2: RNA-seq data analysis. Quality Control. Read mapping. Data Normalization and Exploratory Analysis. Differential gene expression analysis. Functional Enrichment Analysis.
• Unit 3: ChIP-seq data analysis. Quality control. Read mapping. Peak calling and annotation. Data Normalization and Exploratory Analysis. Differential analysis of histone modification.
• Unit 4: Integrative Analysis. Association between histone modifications and gene expression. Weighted Gene Co-expression Networks. Transcriptional Gene Regulatory Networks.

Duration: 20 h
09.00 – 10.30: First Period
10.30 – 11.00: Coffee Break
11.00 – 12.30: Second Period
12.30 – 14.00: Lunch Break
14.00 – 16.00: Third Period

Number of trainees: 20 (10 groups of two people working together)