Human activities such as industry and agriculture have resulted in the high contamination of soil and water with heavy metals. This type of contamination induces harmful effects on environmental and human health. In the last years, research on phytoremediation has highlighted the scientific and economic benefits of plants and their associated microorganisms used for the reclamation of polluted soil and water. Also, the potential of microorganisms to enhance phytoremediation processes and the exact mechanism by which bacteria could enhance heavy metal accumulation in plants has been received some attention. Despite the great interest in improving the efficiency of metal hyperaccumulating plants, their influence on microbial community’s remains poorly elucidated, constituting a less explored aspect in phytoremediation processes. The Estarreja region’s soils are a hotspot to study the impact of phytoremediation in the structure and diversity of microbial communities inhabiting sites contaminated with heavy metals. This project will focus on soil contaminated with Cd and Pb that although less abundant have serious implications for human health.The present study aimed to assess the impact of the plants on the bacterial communities along phytoremediation process in Cd and Pb-contaminated soil from Estarreja region.